BD™ Cytometric Bead Array (CBA) Human Th1/Th2/Th17 CBA Kit
(RUO)
Resolution of the individual bead populations in the BD CBA Human Th1/Th2/Th17 Cytokine Kit. Each population corresponds to an individual analyte.
Resolution of the individual bead populations in the BD CBA Human Th1/Th2/Th17 Cytokine Kit. Each population corresponds to an individual analyte.
Product Details
BD™ Cytometric Bead Array (CBA)
Human (QC Testing)
Flow cytometry (Routinely Tested)
RUO
AB_2869353
Preparation And Storage
Store all kit components at 2 to 8°C. Do not freeze.
560484 Rev. 1
Components
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Human IL-2 Capture Beads | 80 Tests (1 ea) | 51-9005736 | N/A |
| Human IL-10 Capture Beads | 80 Tests (1 ea) | 51-9005740 | N/A |
| Human TNF Capture Beads | 1 Each (1 ea) | 51-9005724 | N/A |
| Human IL-4 Capture Beads | 1 Each (1 ea) | 51-9005715 | N/A |
| Human IFN-γ Capture Beads | 1 Each (1 ea) | 51-9005727 | N/A |
| Human IL-6 Capture Beads | 80 Tests (1 ea) | 51-9005738 | N/A |
| Human IL-17A Capture Beads | 100 Tests (1 ea) | 51-9006289 | N/A |
| B Human Th1/Th2/Th17 PE Detection Reagent | N/A | 51-9006291 | N/A |
| C) Human Th1/Th2/Th17 Cytokine Standards | N/A | 51-9006249 | N/A |
| D Cytometer Setup Beads | 1 Each (1 ea) | 51-9005731 | N/A |
| E1 PE Positive Control Detector | 1 Each (1 ea) | 51-9005732 | N/A |
| E2 FITC Positive Control Detector | 1 Each (1 ea) | 51-9005734 | N/A |
| F Wash Buffer | 1 Each (1 ea) | 51-2431KC | N/A |
| G Assay Diluent | 1 Each (1 ea) | 51-2432KC | N/A |
| BD(tm) CBA Serum Enhancement Buffer (H) | N/A | 51-9002150 | N/A |
560484 Rev. 1
Citations & References
Development References (13)
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Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
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Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
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Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
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Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
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Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
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Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
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Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
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Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
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Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
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Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
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McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). View Reference
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Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
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Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
560484 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.