Applications and Techniques

Expertly design your panels with appropriate lasers and fluorochromes to maximize your multicolor flow analysis. Exploit our 45+ years of flow cytometry expertise and powerful tools, such as the Interactive Cell Map, Clone Comparison, and BD^®Spectrum Viewer, to guide and support you through simple-to high-parametric analysis of multiple subpopulations and sample types. Find tips on how to combine the power and capabilities of our flow cytometry instruments, reagents and software and examine sample data to understand their performance.

Explore the capabilities of the fluorescently barcoded BD^® Cytometric Bead Arrays for detecting a variety of cytokines, chemokines, growth factors and phosphorylated signaling proteins and their flexibility for creating novel multiplex panels. Find out more information on our sandwich ELISA options and protocols for specific targets or see how you can analyze individual cells that secrete a particular protein in vitro using our ELISPOT immunoassays. 

Take advantage of the extensive immunofluorescence (IF) tools that BD Biosciences offers, including a comprehensive portfolio of monoclonal antibodies, fluorescent dyes and reagents; and recommendations on filter configurations for various scenarios. Find useful IF protocols and sample data.

Find out how to take advantage of antibodies conjugated to magnetic particles for positive and negative selection and pre-enrichment prior to cell sorting.

Learn how this novel high-speed cell imaging technology amplifies the power of cell sorting and analysis through real-time integration of image and flow cytometry data. Leverage real-time visual inspection of flow events, comprehensive cell characterization and image-based sorting capabilities to get precise cellular insights.